CD172a/b Mouse anti-Human, Brilliant Violet 786, Clone: SE5A5, BD Optibuild™

Catalog No.	Quantity
BDB743567	50 μg

Catalog No. BDB743567 Supplier BD Biosciences Supplier No. 743567

Description

Mouse Monoclonal Antibody

The SE5A5 monoclonal antibody specifically binds to a common epitope on CD172a/ SIRPα (90 kDa) and CD172b/ SIRPβ 1 (50 kDa) . These transmembrane glycoproteins are members of the Signal Regulatory Protein (SIRP) family that, in turn, belongs to the Immunoglobulin superfamily. The SIRP family is comprised of two subgroups, SIRPα and SIRPβ that are distinguished by the presence (α) or absence (β) of a cytoplasmic domain containing two immunoreceptor tyrosine-based inhibition motifs (ITIM). CD172a/ SIRPα is expressed on CD34+ stem/progenitor cells, cardiomyocytes, monocytes, macrophages, granulocytes, dendritic cells, and in the central nervous system. It binds to CD47 and is implicated in mediating inhibitory signals via the ITIM/SHP-2 association. CD172b/ SIRPβ 1 does not possess a cytoplasmic domain but instead the transmembrane domain contains a positively-charged residue that can interact with another transmembrane protein, DAP-12/KARAP. DAP-12 has two immunoreceptor tyrosine-based activat ion motifs (ITAM) within its cytoplasmic domain that are thought to link CD172b to cellular activation signaling . CD172b is expressed on myeloid cells, including peripheral blood monocytes and granulocytes. It is not expressed on CD34+ cells. CD172a and CD172b have complementary role s in signal regulation and may work together in tuning certain cellular responses to stimuli.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter). BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm. BV786 offers a bright choice for the 6th detector off the violet laser.

Specifications

• Antigen: CD172a/b
• Classification: Monoclonal
• Clone: SE5A5
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 786
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: SIRP alpha/beta1, SIRPα/SIRPβ1, Signal Regulatory Protein α/β1
• Host Species: Mouse
• Immunogen: Human SIRP alpha extracellular domain Recombinant Protein
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ