Invitrogen™ CD172a (SIRP alpha) Monoclonal Antibody (OX41), APC, eBioscience™

Catalog No.	Quantity
50-112-3093	100 μg

Catalog No. 50-112-3093 Supplier Invitrogen™ Supplier No. 17172082

Description

Mouse Monoclonal Antibody

Description: The monoclonal antibody OX41 reacts with rat CD172a, which is also known as signal-regulatory protein alpha (SIRPa). SIRP family members are transmembrane glycoproteins with 3 Ig-like extracellular domains and a cytoplasmic tail containing 2 immunocreceptor tyrosine-based inhibition motifs. CD172a is expressed by monocytes, macrophages, granulocytes, dendritic cells, and neurons. SIRPa association with CD47 inhibits phagocytosis of CD47 presenting cells. Studies suggest that SIRPa ligation prevents phagocytosis by inactivating paxillin and cofilin, proteins involved in cytoskeleton remodeling. Applications Reported: This OX41 antibody has been reported for use in flow cytometric analysis. Applications Tested: This OX41 antibody has been tested by flow cytometric analysis of rat lysed whole blood. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and hematopoietic stem cells. SIRP alpha acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. The ligand binding of SIRP alpha to integrin-associated protein CD47 results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of SIRP alpha, which mediates the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. Ligation of SIRP alpha with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs. SIRP alpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion, and plays a general role in different physiological and pathological processes. Cancer cells highly express CD47, which activates SIRP alpha and inhibits macrophage-mediated destruction of cancerous cell growth.

Specifications

• Antigen: CD172a (SIRP alpha)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: OX41
• Concentration: 0.2 mg/mL
• Conjugate: APC
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: SIRPA
• Gene Accession No.: P97710
• Gene Alias: AI835480; Bit; brain Ig-like molecule with tyrosine-based activation motifs; Brain immunoglobulin like protein with tyrosine - based activation motifs; brain immunological-like with tyrosine-based motifs; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; CD172; CD172 alpha; CD172 antigen-like family member A; CD172A; Inhibitory receptor SHPS-1; macrophage fusion receptor; Macrophage membrane protein MFP150; MFR; mSIRP-alpha1; Myd1; MYD-1; myD-1 antigen; p84; Protein tyrosine phosphatase non-receptor type substrate 1 (SHP substrate 1); protein tyrosine phosphatase, non-receptor type substrate 1; Protein tyrosine phosphatase, non-receptor type substrate 1 (SHP substrate 1); Ptpns1; SHP substrate 1; SHP-1; SHPS1; SHPS-1; signal regulatory protein alpha; signal-regulatory protein alpha; signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; SIRP; Sirpa; SIRPalpha; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3; swc3; swine workshop cluster 3 antigen; swine workshop cluster 3 antigen precursor; tyrosine phosphatase SHP substrate 1; tyrosine-protein phosphatase non-receptor type substrate 1
• Gene Symbols: SIRPA
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 25528
• Target Species: Rat
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ