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Invitrogen™ CD177 Monoclonal Antibody (MEM-166 (MEM166)), eFluor™ 660, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 50177942

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Catalog No. 50-177-942


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Description

Description

Description: The MEM-166 monoclonal antibody reacts with human CD177, a 58-64 kDa GPI-anchored glycoprotein. The human CD177 locus codes for at least two alleles, NB1 and PRV-1, which differ by only 4 amino acids. CD177 is expressed on granulocytes, early erythroblasts, megakaryocytes, promyelocytes and myelocytes. CD177 mRNA levels are elevated in several different myeloproliferative disorders including polycythemia vera, and antibodies to CD177 are involved in neonatal alloimmune neutropenia, autoimmune drug induced neutropenia, and graft failure following marrow transplantation. Applications Reported: This MEM-166 (MEM166) antibody has been reported for use in flow cytometric analysis. Applications Tested: This MEM-166 (MEM166) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.015 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).
TRUSTED_SUSTAINABILITY
Specifications

Specifications

CD177
Monoclonal
5 μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
Q8N6Q3
CD177
Affinity chromatography
RUO
57126
4°C, store in dark, DO NOT FREEZE!
Liquid
Flow Cytometry
MEM-166 (MEM166)
eFluor 660
CD177
1190003K14Rik; CD177; CD177 antigen; CD177 molecule; cell surface receptor; HNA2A; HNA-2a; Human neutrophil alloantigen 2a; NB1; NB1 glycoprotein; NB1 GP; Pdp3; Polycythemia rubra vera protein 1; PRV1; PRV-1; RGD1562941; UNQ595/PRO1181
Mouse
100 Tests
Primary
Human
Antibody
IgG1 κ
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