Invitrogen™ CD18 (LFA-1 beta) Monoclonal Antibody (6.7), FITC, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 11018942

Catalog No. 50-245-229

Description

Description: The 6.7 monoclonal antibody reacts with human and non-human primate (rhesus or cynomologus) CD18, which is also known as beta2 integrin. CD18 is a 90-95 kDa type I transmembrane protein that heterodimerizes with the a chain of CD11a, CD11b and CD11c. The CD18/CD11a complex is also known as lymphocyte function-associated antigen-1 (LFA-1) and plays an important role in cellular adhesion and inflammatory reactions. CD18 is expressed broadly by human lymphocytes and monocytes. Peripheral blood granulocytes also express CD18, but at lower levels. The 6.7 monoclonal antibody has been reported to induce the proliferation of resting T cells cultured with monocytes. Applications Reported: This 6.7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 6.7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leucocytes. CD18 can combine with integrin molecules CD11a-c to form heterodimers at the cell surface, and these heterodimers are known to participate in the process of cell adhesion as well as cell-surface mediated signaling. CD18 forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits, and these integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. Defects in the CD18 gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the CD18 protein have been identified.

Specifications

• Antigen: CD18 (LFA-1 beta)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P05107
• Gene Symbols: ITGB2
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3689
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 6.7
• Conjugate: FITC
• Gene: ITGB2
• Gene Alias: 2E6; AI528527; antigen CD18; B-2 integrin; CD18; CD18 leukocyte adhesion molecule; CD18 subunit; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor); cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; complement component 3 receptor 3 and 4 subunit; complement receptor C3 beta-subunit; complement receptor C3 subunit beta; integrin beta 2; integrin beta chain, beta 2; integrin beta-2; integrin subunit beta 2; integrin, beta 2; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; macrophage antigen 1 (mac-1) beta subunit); integrin, beta 2 (antigen CD18 subunit (p95), lymphocyte function-associated antigen 1, integrin B2); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGB2; LAD; LCAMB; Leu-CAM receptor; leukocyte cell adhesion molecule CD18; leukocyte surface protein; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; Lfa1; LFA-1; lymphocyte function associated antigen 1; MAC-1; Mac-1 beta; macrophage antigen 1 (mac-1) beta subunit); macrophage antigen-1 beta; MF17; MFI7
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human, Monkey
• Product Type: Antibody
• Isotype: IgG1 κ

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov