CD18 Rat anti-Mouse, Brilliant Violet 421, Clone: M18/2, BD Optibuild
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 744597
The M18/2 antibody specifically recognizes the commonβ2 chain of LFA-1 (CD11a/CD18,αLβ2 integrin), Mac-1 (CD11b/CD18,αMβ2 integrin), and gp150, 95 (CD11c/CD18,αXβ2 integrin). Expression of CD18 is limited to leukocytes, where it is widely distributed in consort with the three integrinαchains (CD11a, CD11b, and CD11c). Among splenocytes, NK cells have the highest density of CD18, and T lymphocytes express a higher density than the remaining cells. Theβ2 integrins are important mediators of leukocyte-endothelium interactions. It has been reported that M18/2 antibody blocks in vivo /metastasis of the LB lymphoma to the spleen and that it blocks in vitro formation of aggregates of LB cells and splenocytes. However, other reports indicate that mAb M18/2 has no effect on CTLmediated killing, adherence of C3bi-sensitized erythrocytes to Mac-1, antigen-specific binding of T cells to antigen-producing cells, or rejection of cardiac allografts. Recent in vitro studies indicate that M18/2 antibody stimulates adhesion of Mac-1 to its ligands C3bi and ICAM-1, and it stimulates adhesion of LFA-1 to ICAM-1, but it has no effect upon the interactions of LFA-1 with ICAM-2 nor ICAM-3.The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|Cd18, ITGB2, Integrin β2 chain|
|Mouse CTL glycoproteins|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.|
|Brilliant Violet 421|
|Rat IgG2a, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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