Invitrogen™ CD19 Monoclonal Antibody (6OMP31), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Catalog No.	Quantity
53-019-482	100 μg
53-019-480	25 μg

Catalog No. 53-019-482 Supplier Invitrogen™ Supplier No. 53019482

Description

Rat Monoclonal Antibody

Description: The 6OMP31 monoclonal antibody reacts with human, mouse, and rat CD19. CD19 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD19 was first identified as the B4 antigen of human B lymphocytes using the anti-B4 monoclonal. It is expressed by B cells during all stages of development, except on terminally differentiated plasma cells, and on follicular dendritic cells. CD19 acts primarily as a B cell co-receptor and together with CD21, CD81, Leu13, and MHC class II forms a multi-molecular complex that associates with the BCR. CD19 plays a role in the development, activation, differentiation, and proliferation of B cells. CD19 deficiency leads to an overall impaired humoral immune response. Mutations in CD19 have been associated with severe immunodeficiency syndromes. Although it is unclear whether CD19 contributes directly to B cell carcinogenesis, it is expressed on most B cell tumors, such as acute lymphoblastic leukemias and B cell lymphomas. The use of CD19 monoclonal antibodies for therapies against lymphoma, leukemia and autoimmune disorders are currently being explored. Applications Reported: This 6OMP31 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

CD19 is a member of the immunoglobulin superfamily, characterized by two Ig-like domains, and is expressed on B cells throughout all stages of development, excluding terminally differentiated plasma cells. It is also expressed on follicular dendritic cells and has been observed on myeloid leukemia cells, particularly those of monocytic lineage. CD19 is considered the earliest and broadest B cell-restricted antigen, and its expression is found in all B cell precursor leukemias. CD19 forms a multimolecular complex with CD21, CD81, Leu13, MHC class II, and the B cell receptor (BCR), playing a crucial role in B cell signaling. As a signal-amplifying coreceptor for the BCR, CD19 lowers the threshold for antigen receptor-dependent stimulation, allowing B cells to respond specifically and sensitively to various antigens through low-affinity antigen receptors. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells. Beyond its role as a BCR coreceptor, CD19 can also signal independently of BCR co-ligation, serving as a central regulatory component upon which multiple signaling pathways converge. This makes CD19 an important functional regulator of both normal and malignant B cell proliferation. Mutations in the CD19 gene can result in hypogammaglobulinemia, a condition characterized by low levels of immunoglobulins, while CD19 overexpression can lead to B cell hyperactivity. CD19 is expressed on 100% of peripheral B cells, as defined by the expression of kappa or lambda light chains, underscoring its significance in B cell function and immune regulation.

Specifications

• Antigen: CD19
• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: 6OMP31
• Concentration: 0.5 mg/mL
• Conjugate: Alexa Fluor 488
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: Cd19
• Gene Accession No.: P15391, P25918
• Gene Alias: AW495831; B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Cd19; CD19 antigen; CD19 molecule; CVID3; differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12
• Gene Symbols: Cd19
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12478, 365367, 930
• Target Species: Human, Mouse, Rat
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ

For Research Use Only.