Invitrogen™ CD19 Monoclonal Antibody (HIB19), NovaFluor™ Blue 510, eBioscience™

Catalog No.	Quantity
H004T02B01A	25 Tests
H004T03B01A	100 Tests

Catalog No. H004T02B01A Supplier Invitrogen™ Supplier No. H004T02B01A

Description

Mouse Monoclonal Antibody

Description The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes. This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer. Applications Tested This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (1.0 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Master mixes Master mixes of NFs should be made at 2-8 °C and may be made up to 4 hours ahead of time. We do not recommend storing master mixes containing NovaFluor conjugates overnight or longer. Whole Blood compatibility When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates. See the Bulk Lysis of Human Whole Blood protocol here. Staining of whole blood with NovaFluor conjugates followed by lysis of red blood cells may result in higher-than-expected background staining. Viability dye compatibility NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use v...

CD19 is a member of the immunoglobulin superfamily, characterized by two Ig-like domains, and is expressed on B cells throughout all stages of development, excluding terminally differentiated plasma cells. It is also expressed on follicular dendritic cells and has been observed on myeloid leukemia cells, particularly those of monocytic lineage. CD19 is considered the earliest and broadest B cell-restricted antigen, and its expression is found in all B cell precursor leukemias. CD19 forms a multimolecular complex with CD21, CD81, Leu13, MHC class II, and the B cell receptor (BCR), playing a crucial role in B cell signaling. As a signal-amplifying coreceptor for the BCR, CD19 lowers the threshold for antigen receptor-dependent stimulation, allowing B cells to respond specifically and sensitively to various antigens through low-affinity antigen receptors. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells. Beyond its role as a BCR coreceptor, CD19 can also signal independently of BCR co-ligation, serving as a central regulatory component upon which multiple signaling pathways converge. This makes CD19 an important functional regulator of both normal and malignant B cell proliferation. Mutations in the CD19 gene can result in hypogammaglobulinemia, a condition characterized by low levels of immunoglobulins, while CD19 overexpression can lead to B cell hyperactivity. CD19 is expressed on 100% of peripheral B cells, as defined by the expression of kappa or lambda light chains, underscoring its significance in B cell function and immune regulation.

Specifications

• Antigen: CD19
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: HIB19
• Concentration: 1.0 μg/Test
• Conjugate: NovaFluor Blue 510
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene: Cd19
• Gene Accession No.: P15391
• Gene Alias: AW495831; B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Cd19; CD19 antigen; CD19 molecule; CVID3; differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12
• Gene Symbols: Cd19
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 25 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 930
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Format: Liquid
• Isotype: IgG1 κ

Frequently Asked Questions (FAQs)

What fixation methods are compatible with NovaFluor dyes?

NovaFluor conjugates have been tested to be stable for up to 14 days on cells fixed with 1% formalin, 2% paraformaldehyde, eBioscience IC Fixation Buffer (Cat. No. 00-8222-49), or eBioscience Foxp3 / Transcription Factor Staining Buffer Set (Cat. No. 00-5523-00) when stored at 2-8 degrees C in the dark.

NovaFluor dyes can also be used as a surface stain prior to methanol fixation.