Invitrogen™ CD195 (CCR5) Monoclonal Antibody (eBioT21/8 (T21/8)), eBioscience™, Invitrogen™

Catalog No.	Quantity
14-195-737	2 mg

Catalog No. 14-195-737 Supplier Invitrogen™ Supplier No. 14195737

Description

Mouse Monoclonal Antibody

The eBioT21/8 MAb reacts with the amino terminus of human CCR5 (CD195), an approximately 45 kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1alpha, MIP-1beta and RANTES. Human CCR5 is expressed on resting T cells with a memory/effector phenotype, monocytes, macrophages and immature dendritic cells. CCR5 functions as a receptor to direct the chemotaxis of lymphocytes, and is the primary co-receptor for macrophage-tropic Human Immunodeficiency Virus Type 1 (HIV-1), which binds CCR5 through gp120. Binding of the eBioT21/8 MAb displays donor variability and may be affected by sub-optimal staining conditions.

Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells. CCR5 is required for the infection by HIV-1, HIV-2, and SIV. The beta-chemokines RANTES, MIP-alpha and MIP-beta are the ligands for CCR5 and prevent infection by M-tropic HIV-1. CCR5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.

Specifications

• Antigen: CD195 (CCR5)
• Applications: ELISA, Flow Cytometry, Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: eBioT21/8 (T21/8)
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: CCR5
• Gene Accession No.: P51681
• Gene Alias: AM4-7; C-C chemokine receptor type 5; C-C CKR-5; C-C motif chemokine receptor 5 (gene/pseudogene); C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5; Ccr5; CCR-5; CD195; CD195 antigen; chemokine (C-C motif) receptor 5; chemokine (C-C) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; Cmkbr5; HIV-1 fusion coreceptor; IDDM22; MIP-1 alpha receptor
• Gene Symbols: CCR5
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 2 mg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 1234
• Target Species: Human
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ

Safety and Handling

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