CD195 (CCR5) Monoclonal Antibody (HM-CCR5 (7A4)), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-3789	Invitrogen™ 46195180	25 μg
50-112-3790	Invitrogen™ 46195182	100 μg

Description

Description: CCR5 is the major co-receptor for macrophage-tropic isolates of HIV-1. CCR5, also known as CD195, is a seven transmembrane chemokine receptor capable of binding RANTES, MIP-1 alpha and MIP-1 beta. Knockout mice show a decreased recruitment of macrophages and increased susceptibility to infection. Protein and mRNA has been found in macrophages from thioglycolate-treated and activated T cells. The HM-CCR5(7A4) monoclonal antibody binds the N terminal extracellular domain of mouse CCR5 with no crossreactivity to human CCR5. Applications Reported: This HM-CCR5 (7A4) antibody has been reported for use in flow cytometric analysis. Applications Tested: This HM-CCR5 (7A4) antibody has been tested by flow cytometric analysis of mouse thioglycolated- induced peritoneal exudate cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

CD195 (CCR5) Monoclonal antibody specifically detects CD195 (CCR5) in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD195 (CCR5)
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: AM4-7; C-C chemokine receptor type 5; C-C CKR-5; C-C motif chemokine receptor 5 (gene/pseudogene); C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5; Ccr5; CCR-5; CD195; CD195 antigen; chemokine (C-C motif) receptor 5; chemokine (C-C) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; Cmkbr5; HIV-1 fusion coreceptor; IDDM22; MIP-1 alpha receptor
• Gene Symbols: CCR5
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: CCR5

• Clone: HM-CCR5 (7A4)
• Applications: Flow Cytometry
• Conjugate: PerCP-eFluor 710
• Host Species: Armenian Hamster
• Target Species: Mouse
• Gene Accession No.: P51682
• Gene ID (Entrez): 12774
• Isotype: IgG
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.