CD20 Antibody (109-3C2), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2447490.02MG

Catalog No. NBP24474900

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence

CD20 Monoclonal specifically detects CD20 in Human samples. It is validated for ELISA, Functional.

Specifications

• Antigen: CD20
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Accession No.: P11836
• Gene Symbols: MS4A1
• Immunogen: Stimulated human leukocytes
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a protein of 30-33kDa, which is identified as CD20 (Workshop V; Code CD20.12. Workshop IV; Code B17). It recognizes an extracellular domain of CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. The protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as calcium channel involved in B cell activation and cell cycle progression.
• Content And Storage: Store at 4C.
• Isotype: IgG3 κ

• Applications: Flow Cytometry, Immunofluorescence
• Clone: 109-3C2
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: B1, B-lymphocyte antigen CD20, B-lymphocyte cell-surface antigen B1, B-lymphocyte surface antigen B1, Bp35MGC3969, CD20 antigen, CD20 receptor, CD20S7, CVID5, LEU-16, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, membrane-spanning 4-domains, subfamily A, member 1, MS4A2
• Host Species: Mouse
• Molecular Weight of Antigen: 35 kDa
• Quantity: 0.02 mg
• Research Discipline: Adaptive Immunity, Cancer Stem Cells, Cell Biology, Cytokine Research, Immunology, Signal Transduction, Stem Cell Markers, Tumor Biomarkers
• Gene ID (Entrez): 931
• Target Species: Human
• Form: Purified

For Research Use Only