Invitrogen™ CD200R3 Monoclonal Antibody (Ba103), FITC

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MA549007

Catalog No. FERMA549007

Description

The monoclonal antibody Ba103 recognizes mouse CD200 Receptor-like 3 (CD200R3). Monoclonal antibody Ba103 activates basophils ex vivo and elicits systemic anaphylaxis when administered in vivo. Cross-linking of CD200R3 on MC/9 cells by monoclonal antibody Ba103 induces degranulation. Furthermore, monoclonal antibody Ba103 is useful for depletion of basophils. This antibody does not cross-react with CD200R, CD200R2, CD200R4 or CD200R5.

CD200R3 is a dimeric cell surface receptor also known as CD200RLb. CD200R3 contains two Ig-like extracellular domains and as such is a member of the immunoglobulin superfamily of receptors. The intracellular region of CD200R3 associates with the adaptor molecule DAP12, and possibly also DAP10, CD3 zeta and FcR gamma-chain, indicating that it is capable of transducing stimulatory signals. In this respect CD200R3 bears similarity to activatory receptors CD200R2 and CD200R4. In contrast, an inhibitory receptor CD200R (also known as CD200R1) relies on other adaptors. CD200R3 is primarily expressed by basophils and mast cells, cells known to be involved in anti-parasitic immune responses and frequently also allergic reactions. The surface expression of CD200R3 decreases when basophils are activated by ligating their CD16 and CD32. Remarkably, most of this downregulation is observed within the first 30 minutes after activation. Unlike CD200R, CD200R3 does not bind CD200.

Specifications

• Antigen: CD200R3
• Classification: Monoclonal
• Concentration: 100 μg/mL
• Formulation: PBS with 1% BSA and 0.02% sodium azide
• Gene Accession No.: Q5UKY4
• Gene Symbols: CD200R3
• Immunogen: Mouse primary basophils; cell-enriched bone marrow cells.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Clone: Ba103
• Conjugate: FITC
• Gene: CD200R3
• Gene Alias: 4733401I18Rik; 4833409J19Rik; AI505817; BB106877; CD200 cell surface glycoprotein receptor 3; CD200 cell surface glycoprotein receptor-like 3; CD200 cell surface glycoprotein receptor-like b; CD200 receptor 3; CD200 receptor-like 3; Cd200r3; CD200RLb; cell surface glycoprotein CD200 receptor 3; Cell surface glycoprotein OX2 receptor 3; mCD200RLb
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 74603
• Content And Storage: 4°C, store in dark
• Form: Liquid