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Invitrogen™ CD218a (IL-18Ra) Monoclonal Antibody (P3TUNYA), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Description
IL18R1 is a cytokine receptor that belongs to the interleukin 1 receptor family. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. The gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q.
Specifications
Specifications
| Antigen | CD218a (IL-18Ra) |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | P3TUNYA |
| Concentration | 0.2 mg/mL |
| Conjugate | PerCP-eFluor 710 |
| Formulation | PBS with 0.09% sodium azide; pH 7.2 |
| Gene | Il18r1 |
| Gene Accession No. | Q61098 |
| Gene Alias | CD218 antigen-like family member A; CD218a; CDw218a; cytokine receptor; IL1 receptor-related protein; Il18r1; IL-18R1; IL-18R-1; IL18RA; Il18ralpha; IL-18R-alpha; IL18Ralpha2; IL1RRP; IL-1Rrp; IL1R-rp; interleukin 1 receptor related protein; interleukin 18 receptor 1; Interleukin-18; interleukin-18 receptor 1; Interleukin-18 receptor alpha |
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Frequently Asked Questions (FAQs)
Our options will depend on the samples you are analyzing.
If cell viability is not critical, you can store your stained samples at 4 degrees C or on ice overnight in the dark and analyze the following day.
For samples stained with eFluor organic fluorochromes, we recommend that cells be suspended in 100 uL of Flow Cytometry Staining Buffer (Cat. No. 00-4222) and 100 uL of eBioscience IC Fixation Buffer (Cat. No. 00-8222); samples can be incubated for up to 3 days at 4 degrees C in the dark. Alternatively, the 1-step Fix/Lyse Solution (Cat. No. 00-5333) can be used. This is a great option when working with whole blood but also works for other cell types.
Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).
The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.
The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.
As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.
Safety and Handling
For Research Use Only.
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