CD22 Mouse anti-Human, Clone: OTI2E6, lyophilized, TrueMAB™

Catalog No.	Quantity
50-166-8509	100 μg

Catalog No. 50-166-8509 Supplier OriGene Supplier No. CF807826

Description

Mouse Monoclonal Antibody

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product No. 7KMWCO

CD22 (BL-CAM) is a type 1 integral membrane glycoprotein with molecular weight of 130 to 140 kDa. CD22 is expressed in both the cytoplasm and cell membrane of B-lymphocytes. CD22 antigen appears early in B-cell lymphocyte differentiation at approximately the same stage as the CD19 antigen. Unlike other B-cell markers, CD22 membrane expression is limited to the late differentiation stages comprised between mature B cells (CD22+) and plasma cells (CD22-), and may thus prove useful in phenotyping mature leukemias. CD22 is also strongly expressed in hairy cell leukemia. CD22 preferentially binds to alpha2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B cell antigen receptor signaling. CD22 plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. CD22 is also strongly expressed in hairy cell leukemia. CD22 is also positive in diffuse large B-cell lymphoma and nodular lymphocyte predomice Hodgkin′s lymphoma, but negative in classical Hodgkin′s lymphoma.

Specifications

• Antigen: CD22
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: OTI2E6
• Conjugate: Unconjugated
• Formulation: PBS with 8% trehalose and no preservative; pH 7.3
• Gene: CD22
• Gene Accession No.: P20273
• Gene Alias: SIGLEC-2, SIGLEC2
• Gene Symbols: CD22
• Host Species: Mouse
• Immunogen: Human recombit protein fragment corresponding to amino acids 707-847 of human CD22 produced in E.coli.
• Purification Method: Affinity Chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 933
• Target Species: Human
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG2b