Invitrogen™ CD22 Polyclonal Antibody

Goat Polyclonal Antibody

Supplier:  Invitrogen™ PA547292

Catalog No. PIPA547292

Description

In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Siglec-2 is observed and less than 1% cross-reactivity with rhSiglec-5, rhSiglec-7, rhSiglec-9, rhSiglec-10, recombinant mouse (rm) Siglec-3, and rmSiglec-F is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is <0.10 EU per 1 μg of the antibody by the LAL method.

CD22 (BL-CAM) is a type 1 integral membrane glycoprotein with molecular weight of 130 to 140 kDa. CD22 is expressed in both the cytoplasm and cell membrane of B-lymphocytes. CD22 antigen appears early in B-cell lymphocyte differentiation at approximately the same stage as the CD19 antigen. Unlike other B-cell markers, CD22 membrane expression is limited to the late differentiation stages comprised between mature B cells (CD22+) and plasma cells (CD22-), and may thus prove useful in phenotyping mature leukemias. CD22 is also strongly expressed in hairy cell leukemia. CD22 preferentially binds to alpha2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B cell antigen receptor signaling. CD22 plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. CD22 is also strongly expressed in hairy cell leukemia. CD22 is also positive in diffuse large B-cell lymphoma and nodular lymphocyte predominance Hodgkin's lymphoma, but negative in classical Hodgkin's lymphoma.

Specifications

• Antigen: CD22
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CD22
• Gene Alias: A530093D23; B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; B-lymphocyte cell adhesion molecule (BL-CAM); Cd22; CD22 antigen; CD22 molecule; FLJ22814; Lectin 2; Leu-14; Lyb8; Lyb-8; MGC130020; sialic acid binding Ig-like lectin 2; sialic acid-binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2 (Siglec-2); Siglec2; Siglec-2; T-cell surface antigen Leu-14
• Host Species: Goat
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12483
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: Neutralization, Western Blot, Immunocytochemistry
• Concentration: 0.2 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: P35329
• Gene Symbols: CD22
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Siglec-2/CD22 Ser22-Arg702.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG