CD229 (Ly-9) Monoclonal Antibody (HLy9.25), APC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 17229942

Catalog No. 50-151-32

Description

Description: This HLy9.25 monoclonal antibody reacts with human CD229 (also known as Ly9 or SLAMF3), an approximately 120-kDa member of the Signaling Lymphocyte Activation Molecule (SLAM) immunoglobulin superfamily. This type I transmembrane glycoprotein is expressed on a variety of immune cell types, including thymocytes, T and B lymphocytes, and natural killer cells. Expression of CD229 is also detected on T and B cell malignancies, including Jurkat and Burkitt lymphoma cells, respectively. CD229 has been shown to interact with SH2-binding phosphatases and the adaptor proteins SLAM-associated protein (SAP) and EAT-2. Moreover, CD229 localizes to the immunological synapse following T cell activation. Applications Reported: This HLy9.25 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HLy9.25 antibody has been pre-titrated and tested by flow cytometric analysis on normal human peripheral blood cells. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.

Specifications

• Antigen: CD229 (Ly-9)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9HBG7
• Gene Symbols: Ly9
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 4063
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: HLy9.25
• Conjugate: APC
• Gene: Ly9
• Gene Alias: AI893573; CD229; CDABP0070; Cell surface molecule Ly-9; hly9; Lgp100; LY9; Ly-9; Lymphocyte antigen 9; mLY9; RP11-312J18.1; Signaling lymphocytic activation molecule 3; SLAM family member 3; SLAMF3; T100; T-lymphocyte surface antigen Ly-9
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.