CD23/Fc epsilon RII Rat anti-Mouse, Alexa Fluor 594, Clone: 691632, R&D Systems™

Catalog No.	Quantity
FAB6900T025	25 Tests

Catalog No. FAB6900T025 Supplier R&D Systems Supplier No. FAB6900T025

Description

Rat Monoclonal Antibody

The low affinity receptor for IgE, Fcε RII (designated CD23) is a member of subgroup II of the C-type (Ca²⁺-dependent) lectin superfamily (1-4). Mouse CD23 is a45-49kDa type II transmembrane glycoprotein that binds mouse, but not human, IgE (1-4). The longest isoform is 331 amino acids (aa) in length and contains a 23 aa cytoplasmic domain, a 26 aa transmembrane segment, and a 282 aa extracellular domain (ECD). The ECD C-type lectin domain binds both protein and carbohydrate, with separate sites for binding IgE, CD21, and β2 and αv integrins (2). Coiled-coil topology within a connecting stalk contributes to oligomerization, which increases IgE affinity (1, 2). Mouse CD23 shares 88% aa sequence identity with rat CD23 and up to 57% aa identity with isoforms of human, equine, porcine and bovine CD23. Isoforms which vary in the cytoplasmic sequence include the “a” isoform, which begins with the sequence MEENEYS and is constitutively expressed by B cells, and the cb” isoform, which begins with MDTHHT and is induced by IL-4 on a variety of inflammatory cells, B cells and epithelia (2, 5-7). The isoforms differentially participate in IgE-mediated endocytosis and phagocytosis (2, 8-10). CD23b and two 309 aa mouse CD23b isoforms, lacking either exon 5 or 6, also display distinct endocytic properties on intestinal epithelia (6, 11). Several soluble forms of CD23 are mainly generated by metalloprotease (especially ADAM10) and cysteine protease activity, although potentially soluble mouse isoforms have also been sequenced (12-14). Both soluble and membrane-bound forms of CD23 include the lectin domain and show bioactivity. CD23 binding to monocyte integrins results in oxidative product generation and proinflammatory cytokine release (15). On human, but not mouse, B cells, sCD23 induces IgE secretion by binding CD21 (2, 9). In both, secreted IgE will bind B cell membrane CD23, rendering it unavailable for cleavage, and thus shutting down IgE production (2).

Specifications

• Antigen: CD23/Fc epsilon RII
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 691632
• Concentration: 0.2 mg/mL
• Conjugate: Alexa Fluor 594
• Dilution: Flow Cytometry 5 uL/10^6 cells
• Formulation: Supplied in a saline solution containing BSA and Sodium Azide. with Sodium Azide
• Gene Accession No.: P20693
• Gene Alias: BLAST-2, CD23, CD23A, CD23CD23 antigen, CLEC4J, CLEC4JC-type lectin domain family 4 member J, C-type lectin domain family 4, member J, Fc epsilon RII, Fc fragment of IgE, low affinity II, receptor for (CD23), FCE2Fc fragment of IgE, low affinity II, receptor for (CD23A), fc-epsilon-RII, FCER2, Fcer2a, FceRII, IGEBF, Immunoglobulin E-binding factor, low affinity immunoglobulin epsilon Fc receptor, Ly-42, Lymphocyte IgE receptor
• Gene Symbols: Fcer2a
• Host Species: Rat
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD23/Fc epsilon RII Glu50-Pro331 Accession # P20693
• Purification Method: Protein A or G purified from hybridoma culture supernatant
• Quantity: 25 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 2208
• Test Specificity: Detects mouse CD23/Fc epsilon RII in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Fc epsilon RIA or rhFc epsilon RIG is observed.
• Target Species: Mouse
• Content And Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
• Form: Purified
• Isotype: IgG2a