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CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), APC-eFluor™ 780, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  LIFE TECHNOLOGIES 47998741

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Catalog No. 47-998-741


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Description

Description

Description: The HIR2 monoclonal antibody reacts with human glycophorin A, sialoglycoproteins expressed by erythroid precursors and mature circulating red cells. Applications Reported: This HIR2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIR2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser

CD235a (Glycophorin A) Monoclonal antibody specifically detects CD235a (Glycophorin A) in Human samples. It is validated for Flow Cytometry.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

CD235a (Glycophorin A)
Monoclonal
5 μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P02724
GYPA
Affinity chromatography
RUO
2993
4° C, store in dark, DO NOT FREEZE!
Liquid
Flow Cytometry
HIR2 (GA-R2)
APC-eFluor 780
GYPA
AI853584; CD235; CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A; glycophorin A (MN blood group); glycophorin A (MNS blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GPSAT; Gypa; HGNC:4702; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); HGpStaC; Mi.V glycoprotein; Mi.V glycoprotein (24 AA); MN; MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR; sialoglycoprotein alpha
Mouse
25 Tests
Primary
Human
Antibody
IgG2b κ
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