Invitrogen™ CD24 Monoclonal Antibody (M1/69), NovaFluor™ Yellow 590, eBioscience™

Catalog No.	Quantity
M014T02Y02A	25 μg

Catalog No. M014T02Y02A Supplier Invitrogen™ Supplier No. M014T02Y02A

Description

Rat Monoclonal Antibody

The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). The expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer. Applications Tested The M1/69 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.8 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Master mixes Master mixes of NFs should be made at 2-8 °C and may be made up to 4 hours ahead of time. We do not recommend storing master mixes containing NovaFluor conjugates overnight or longer. Whole Blood compatibility When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates. See the Bulk Lysis of Human Whole Blood protocol here. Staining of whole blood with NovaFluor conjugates followed by lysis of red blood cells may result in higher-than-expected background staining. Viabili...

CD24 is a 35-50 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on a variety of cell types, including erythrocytes, thymocytes, peripheral lymphocytes, and cells of the myeloid lineage. It is variably glycosylated, resulting in heterogeneity in molecular mass across different cell lineages, which can affect antibody staining levels on lymphocyte populations. In the context of B-cell development, CD24 is expressed from the pro-B-cell stage in the bone marrow through to mature, surface Ig-positive B cells, with very low or negative expression on plasma cells. It is also present on the majority of B-lineage acute lymphoblastic leukemias, B-cell chronic lymphocytic leukemias (CCLs), and B-cell non-Hodgkin's lymphomas. CD24 is thought to play a role in regulating B-cell proliferation and maturation, as well as in controlling autoimmunity. Additionally, CD24 has been reported to interact with P-selectin (CD62P), indicating a potential role in cell adhesion and signaling. CD24 is a valuable marker for studying B-cell development, leukemia, lymphoma, and autoimmune regulation, as well as its interactions with other molecules like P-selectin.

Specifications

• Antigen: CD24
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: M1/69
• Concentration: 0.1 mg/mL
• Conjugate: NovaFluor Yellow 590
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: Cd24a
• Gene Accession No.: P24807
• Gene Alias: CD24; CD24 antigen; CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24 molecule; CD24A; CD24a antigen; cluster of differentiation 24; FLJ22950; FLJ43543; heat stable antigen; heat-stable antigen; HSA; Ly-52; Lymphocyte antigen 52; M1/69-J11D heat stable antigen; MGC75043; Nectadrin; nectadrin heat stable antigen; R13-Ag; Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen; X62 heat stable antigen
• Gene Symbols: Cd24a
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 25 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12484
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Format: Liquid
• Isotype: IgG2b κ