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CD244.1, PerCP-eFluor™ 710, clone: C9.1, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

$161.00 - $384.00

Specifications

Antigen CD244.1
Clone C9.1
Target Species Murine
Conjugate PerCP-eFluor 710
Isotype IgG2b, kappa
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-113-0795 Non-distribution product as customer accommodation. View Documents Invitrogen
46244080
25μg Each for $161.00
Add to cart
 
50-113-0796 Non-distribution product as customer accommodation. View Documents Invitrogen
46244082
100μg Each for $384.00
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Description

Description

This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Description: The monoclonal antibody C9.1 reacts with mouse CD244.1. This receptor is expressed on NK cells, gamma delta T cells, some CD8+ T cells, basophils, and monocytes on most inbred mouse strains except C57BL/6 and C58, which express the CD244.2 allotype. CD244 is a CD2-related receptor belonging to the SLAM family that exists as two different isoforms with opposing functions. CD244 associates with CD48, which is expressed widely on hematopoietic cells, regulates NK cell proliferation, cytotoxicity, and cytokine production. However, the CD244/CD28 interaction between NK and other cell types inhibits NK cytotoxicity, which contributes to self-tolerance. CD244 may enhance or inhibit CD8+ T cell effector function depending on SAP levels.The C9.1 antibody has been reported to have antagonist activity on activated NK cells. Applications Reported: This C9.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This C9.1 antibody has been tested by flow cytometric anaylsis of BALB/c mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2µm post-manufacturing filtered.
Specifications

Specifications

CD244.1
Murine
IgG2b, kappa
Monoclonal
Liquid
Antibody
Store at 2-8°C. Do not freeze. Light-sensitive material.
C9.1
PerCP-eFluor 710
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.

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