Invitrogen™ CD244 (2B4) Monoclonal Antibody (M085)

Catalog No.	Quantity
PIMA547660	100 μL

Catalog No. PIMA547660 Supplier Invitrogen™ Supplier No. MA547660

Description

Mouse Monoclonal Antibody

Clone M085 mouse monoclonal antibody detects a 110 kDa* protein on SDS-PAGE Native immunoblots of human THP1 monocytes. The antibody detects only the native CD244 protein, and the aldehyde-fixed CD244 protein. The antibody works for native western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.

CD244 (Natural killer (NK) cell receptor 2B4/SLAMF4) is an Ig superfamily signaling lymphocyte activation molecule (SLAM) receptor. Like all SLAM family receptors, it has an extracellular segment with two immunoglobulin (Ig)-like domains, and a cytoplasmic domain containing four immunoreceptor tyrosine-based switch motifs. CD244 does not act as a selfligand similar to other SLAM family receptors. It binds CD48, a transmembrane receptor ubiquitously expressed on hematopoietic cells. CD244 activity is controlled by the presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Downstream signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Activation of CD244 stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis. CD244 is involved in the regulation of CD8+ T-cell proliferation, and inhibits inflammatory responses in dendritic cells (DCs). In cancers, CD244 shows increased expression in intratumoral DCs and myeloid suppressor cells, and anti-CD244 therapies may increase infiltrating T-cells and impair tumor growth.

Specifications

• Antigen: CD244 (2B4)
• Applications: ELISA, Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: M085
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene: CD244
• Gene Accession No.: Q9BZW8
• Gene Alias: NKR2B4; SLAMF4
• Gene Symbols: CD244
• Host Species: Mouse
• Immunogen: Clone (M085) was generated from a recombinant protein that included the extracellular region of human CD244 protein.
• Purification Method: Protein G
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 51744
• Target Species: Human
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1