Invitrogen™ CD244 Monoclonal Antibody (eBioPP35 (PP35)), Functional Grade, eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
16-244-981	Invitrogen™ 16244981	50 μg

Description

Description: The eBioPP35 monoclonal antibody reacts with human CD244, also known as 2B4, p38, NK-cell activation-inducing ligand, signaling lymphocyte activation molecule family member 4, and natural killer cell receptor 2B4. Human CD244 is a 38 kD protein expressed on NK cells, a subset of CD8+ T cells, gammadelta T cells, monocytes, basophils, mast cells, and eosinophils. Binding of CD244 to its ligand, CD48, results in NK cell activation. Costaining experiments with eBioC1.7 suggest that both antibodies recognize different epitopes. Applications Tested: This eBioPP35 (PP35) antibody has been tested by flow cytometric analysis of human peripheral blood cells. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD244 (2B4, SLAMF4) is a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via CD244 is thought to modulate NK-cell cytolytic activity. CD244 interacts with SLAMF2, causing the activation of both SLAMF4- and SLAMF2-expressing cells. Patients with systemic lupus erythmatosus have lower than normal levels of SLAMF4 expressed on their NK cells and monocytes, suggesting that SLAMF4 may play a role in the pathology of this autoimmune disease. Diseases associated with CD244 dysfunction include rheumatoid arthritis and lymphoproliferative syndrome. Alternatively spliced transcript variants encoding different isoforms of CD244 have been found for this gene.

Specifications

• Antigen: CD244
• Concentration: 1 mg/mL
• Applications: Flow Cytometry, Functional Assay
• Conjugate: Functional Grade
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: Q9BZW8
• Gene ID (Entrez): 51744
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: eBioPP35 (PP35)
• Content And Storage: 4°C
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG1
• Target Species: Human
• Gene: CD244
• Gene Alias: 2B4; C9.1; CD244; CD244 long isoform; CD244 molecule; Cd244 molecule, natural killer cell receptor 2B4; CD244 natural killer cell receptor 2B4; CD244 short isoform; F730046O15Rik; h2B4; Ly90; NAIL; natural killer cell receptor 2B4; NK cell activation inducing ligand NAIL; NK cell activation-inducing ligand; NK cell receptor 2B4; NK cell type I receptor protein 2B4; NKR Nmrk; NKR2B4; Nmrk; non MHC restricted killing associated; non-MHC restricted killing associated; Signaling lymphocytic activation molecule 4; SLAM family member 4; SLAMF4; transmembrane NK cell receptor 2B4
• Gene Symbols: CD244
• Product Type: Antibody