Invitrogen™ CD247 (CD3 zeta) Monoclonal Antibody (6B10.2), PerCP-eFluor™ 710, eBioscience™

Catalog No.	Quantity
46-247-942	100 Tests

Catalog No. 46-247-942 Supplier Invitrogen™ Supplier No. 46247942

Description

Mouse Monoclonal Antibody

Description: This 6B10.2 monoclonal antibody reacts with human CD247, which is also known as the invariant zeta chain. The CD3 complex is composed of three monomeric chains (gamma, delta, and epsilon) and the invariant zeta chain, which exists as a homodimer or as a heterodimer with the epsilon subunit. Together with the T cell antigen receptor (TCR), the CD3 gamma, delta, epsilon, and zeta chains comprise the TCR/CD3 complex. CD3 zeta is expressed on immature and mature thymocytes, as well as T lymphocytes and NK cells. Signal transduction during T cell activation is mediated by tyrosine phosphorylation of cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) present on the zeta chain. The 6B10.2 monoclonal antibody recognizes a transmembrane epitope of the CD3 zeta chain. Therefore, flow cytometric analysis with this antibody requires intracellular staining. In addition, this antibody has been reported to recognize the zeta chain as a homodimer but not as a monomer. Applications Reported: This 6B10.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 6B10.2 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracell...

CD3z complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules, and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Diseases associated with CD3z include Immunodeficiency 25 and T-B+ Severe Combined Immunodeficiency Due To Cd3delta/Cd3epsilon/Cd3zeta.

Specifications

• Antigen: CD247 (CD3 zeta)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 6B10.2
• Concentration: 5 μL/Test
• Conjugate: PerCP-eFluor 710
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene: CD247
• Gene Accession No.: P20963
• Gene Alias: 4930549J05Rik; A430104F18Rik; antigen CD3Z, zeta polypeptide; AW552088; Cd247; CD247 antigen; CD247 antigen, zeta subunit; Cd247 molecule; Cd3; CD3 antigen, zeta polypeptide; CD3 zeta; CD3 zeta chain; CD3e antigen; CD3-epsilon; Cd3-eta; CD3H; CD3Q; Cd3z; CD3Z antigen, zeta polypeptide (TiT3 complex); Cd3zeta; Cd3-zeta; CD3zeta chain; CD3-zeta/eta; FLJ18683; IMD25; T3E; T3z; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 eta chain; T-cell receptor T3 zeta chain; T-cell receptor zeta chain; T-cell surface glycoprotein CD3 zeta chain; TCR zeta chain; TCRE; Tcrk; TCRZ; TCRzeta
• Gene Symbols: CD247
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 919
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ

Frequently Asked Questions (FAQs)

I am unable to analyze my cells stained with eFluor Organic Dyes today. What options do I have?

Our options will depend on the samples you are analyzing.

If cell viability is not critical, you can store your stained samples at 4 degrees C or on ice overnight in the dark and analyze the following day.

For samples stained with eFluor organic fluorochromes, we recommend that cells be suspended in 100 uL of Flow Cytometry Staining Buffer (Cat. No. 00-4222) and 100 uL of eBioscience IC Fixation Buffer (Cat. No. 00-8222); samples can be incubated for up to 3 days at 4 degrees C in the dark. Alternatively, the 1-step Fix/Lyse Solution (Cat. No. 00-5333) can be used. This is a great option when working with whole blood but also works for other cell types.

Can the eFluor Organic fluorochromes be used for intracellular staining?

Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).

What buffers are compatible with the eFluor Organic fluorochromes and eVolve QDots?

The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.

Are the eFluor Organic Dyes photo-labile?

As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.