CD249 (BP-1) Monoclonal Antibody (6C3), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-3860	Invitrogen™ 46589182	100 μg

Description

Description: The 6C3 monoclonal antibody reacts with mouse BP-1, also known as Ly-51 and 6C3 antigen. BP-1 is a 140 kDa type II transmembrane protein that is present on the surface as a disulfide-linked homodimer. This molecule is an enzyme with aminopeptidase A (APA) activity and is expressed by bone marrow stromal cells, thymic cortical epithelial cells and at early stages of mouse B cell development, on pre-B cells. The low level of BP-1 expression on pre-B cells can be upregulated in the presence of IL-7. Applications Reported: This 6C3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 6C3 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

CD249 (BP-1) Monoclonal antibody specifically detects CD249 (BP-1) in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD249 (BP-1)
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: 6030431M22Rik; Aminopeptidase A; aminopeptidase-A; APA; AP-A; BP1; Bp-1/6C3; BP-1/6C3 antigen; CD249; differentiation antigen gp160; EAP; ENPEP; Glutamyl aminopeptidase; glutamyl aminopeptidase (aminopeptidase A); gp160; Ly51; Ly-51
• Gene Symbols: Enpep
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: Enpep

• Clone: 6C3
• Applications: Flow Cytometry
• Conjugate: PerCP-eFluor 710
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: P16406
• Gene ID (Entrez): 13809
• Isotype: IgG2a κ
• Purification Method: Affinity Chromatography
• Product Type: Antibody

For Research Use Only.