CD25 (IL-2 Receptor α) Mouse anti-Human, RB545, Clone: 2A3, BD Horizon™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 569268

Catalog No. BDB569268

Description

The 2A3 monoclonal antibody specifically binds to human CD25, the low-affinity alpha subunit of the Interleukin-2 Receptor (IL- 2Rα). CD25 associates with CD122 (IL-2Rβ chain) and CD132 (common γ chain or γc) to form the high-affinity signal-transducing IL-2R complex. CD25 is expressed by subsets of thymocytes and peripheral blood lymphocytes including CD4+CD25+ regulatory T cells and memory T cells. CD25 antigen density increases on activated T cells including phytohemagglutinin (PHA)-, concanavalin A (Con A)-, and CD3-activated T lymphocytes. High levels of CD25 can be expressed by T lymphocytes from mixed lymphocyte cultures and by human T-lymphocyte leukemia virus (HTLV)-infected T-lymphocyte leukemia lines, for example, HUT-102. CD25 can also be expressed by activated B cells and macrophages. Recombinant IL-2 blocks the binding of the 2A3 antibody to PHA-activated T lymphocytes.

CD25 (IL-2 Receptor α) Monoclonal antibody specifically detects CD25 (IL-2 Receptor α) in Human samples. It is validated for Flow Cytometry

Specifications

• Antigen: CD25 (IL-2 Receptor α)
• Classification: Monoclonal
• Conjugate: RB545
• Gene Alias: IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55
• Immunogen: Human Phytohemagglutinin-activated T Cells
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified
• Excitation/Emission: Excitation Source: Blue 488 nm, Excitation Max: 496 nm, Emission Max: 545 nm

• Applications: Flow Cytometry
• Clone: 2A3
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 3559
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
• Isotype: IgG1 κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov