CD25/IL-2R alpha Antibody (143-13), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24508800	Novus Biologicals NBP2450880.02MG	0.02 mg
NBP24508801	Novus Biologicals NBP2450880.1MG	0.1 mg
NBP24508802	Novus Biologicals NBP2450880.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence

CD25/IL-2R alpha Monoclonal specifically detects CD25/IL-2R alpha in Human samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Functional, Immunofluorescence.

Specifications

• Antigen: CD25/IL-2 R alpha
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry, SDS-Page, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Adaptive Immunity, Apoptosis, Cytokine Research, Diabetes Research, Immunology, Innate Immunity, Mast Cell Markers, Signal Transduction
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: CD25, CD25 antigen, IDDM10, IL-2 receptor subunit alpha, IL2R, IL-2R subunit alpha, IL-2-RA, IL2-RA, interleukin 2 receptor, alpha, interleukin-2 receptor subunit alpha, p55, TAC antigen, TCGFR
• Gene Symbols: IL2RA
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes a protein of 55kDa, identified as CD25. It is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkin's disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This MAb recognizes the epitope B, which is located at residue 3-104 of CD25 and doe not block IL-2 binding to CD25.

• Clone: 143-13
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Accession No.: P01589, P01589
• Gene ID (Entrez): 3559
• Immunogen: Stimulated human leukocytes
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 55 kDa

For Research Use Only