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CD27 Monoclonal Antibody (LT27), PE, Invitrogen™

Catalog No. PIMA180114
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100 Tests
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Catalog No. Quantity
PIMA180114 100 Tests
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Catalog No. PIMA180114 Supplier Thermo Scientific Supplier No. MA180114

Description

Mouse Monoclonal Antibody

Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4oC in the dark. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Mouse anti human CD27, clone LT27, recognizes the human T-cell activation antigen, CD27.

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD27
Applications Flow Cytometry
Classification Monoclonal
Clone LT27
Concentration Conc. Not Determined
Conjugate PE
Formulation PBS with 1% BSA, 5% sucrose and 0.09% sodium azide; pH 7.4
Gene CD27
Gene Accession No. P26842
Gene Alias CD antigen 27; CD27; CD27 antigen; CD27 molecule; CD27L receptor; LPFS2; S152; S152. LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T14; T-cell activation antigen CD27; TNFRSF7; TNFSF7; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7
Gene Symbols CD27
Host Species Mouse
Immunogen Normal human blood lymphocytes
Purification Method Protein G
Quantity 100 Tests
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 101114260, 712693, 939
Target Species Human, Ovine, Rhesus Monkey
Content And Storage 4° C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Lyophilized
Isotype IgG2a
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