Invitrogen™ CD27 Recombinant Rabbit Monoclonal Antibody (012), PerCP

Catalog No.	Quantity
PIMA546788	100 Tests

Catalog No. PIMA546788

Supplier: Invitrogen™ MA546788

Description

Rabbit Recombinant Monoclonal Antibody

Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD27 (Met1-Arg182) and conjugated with PerCP under optimum conditions, the unreacted PerCP was removed. Storage: This antibody can be stored at 2C-8C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

Specifications

• Antigen: CD27
• Applications: Flow Cytometry
• Classification: Recombinant Monoclonal
• Clone: 012
• Concentration: 100 μg/mL
• Conjugate: PerCP
• Formulation: PBS with 0.5% BSA and 0.09% sodium azide
• Gene: CD27
• Gene Accession No.: P41272
• Gene Alias: CD antigen 27; CD27; CD27 antigen; CD27 molecule; CD27L receptor; LPFS2; S152; S152. LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T14; T-cell activation antigen CD27; TNFRSF7; TNFSF7; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7
• Gene Symbols: CD27
• Host Species: Rabbit
• Immunogen: Recombinant protein encoding Mouse CD27 (Met1-Arg182).
• Purification Method: Protein A
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 21940
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG