Invitrogen™ CD27 Recombinant Rabbit Monoclonal Antibody (BLR083G)

Catalog No.	Quantity
PIMA544357	100 μL
PIMA544358	10 μL

Catalog No. PIMA544357 Supplier Invitrogen™ Supplier No. MA544357

Description

Rabbit Recombinant Monoclonal Antibody

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

Specifications

• Antigen: CD27
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Recombinant Monoclonal
• Clone: BLR083G
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: BBS with 0.1% BSA and 0.09% sodium azide; pH 7.8-8.6
• Gene: CD27
• Gene Accession No.: P26842
• Gene Alias: CD antigen 27; CD27; CD27 antigen; CD27 molecule; CD27L receptor; LPFS2; S152; S152. LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T14; T-cell activation antigen CD27; TNFRSF7; TNFSF7; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7
• Gene Symbols: CD27
• Host Species: Rabbit
• Immunogen: Recombinant protein representing the extracellular domain of human CD27 (residues 21-187).
• Purification Method: Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 939
• Target Species: Human
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG