Invitrogen™ CD273 (B7-DC) Chimeric Recombinant Rabbit Monoclonal Antibody (TY25)

Catalog No.	Quantity
PIMA548100	200 μg

Catalog No. PIMA548100 Supplier Invitrogen™ Supplier No. MA548100

Description

Rabbit Recombinant Monoclonal Antibody

Specificity: This antibody binds to mouse PD-L2 and competes with PD-1 binding. It does not cross-react with PD-L1.

Programmed death-ligand 2 (PD-L2), or B7-DC, is a member of the B7 ligand family within the immunoglobulin superfamily that, along with programmed death-ligand 1 (PD-L1), acts as a ligand for programmed cell death protein 1 (PD-1). Though expressed primarily in dendritic cells, PD-L2 expression can be induced on a wide variety of immune and non-immune cells depending on the microenvironment. PD-L2 expression is particularly upregulated in the presence of Th2 cytokine, IL-4, as well as Th1 cytokines, TNF-alpha and IFN-gamma to a lesser degree. While generally expressed at lower levels compared to PD-L1, PD-L2 demonstrates a 2 to 6 times higher relative affinity to PD-1 than PD-L1. PD-1 and its ligands are referred to as inhibitory immune checkpoint molecules in that they provide useful negative feedback during physiological homeostasis. Ligation of PD-L2 or PD-L1 inhibits activation, proliferation, and cytokine secretion (e.g. IFN-gamma, IL-10) in T cells, ultimately dampening immune response. Conversely, studies have shown that PD-L2 can also stimulate T cell proliferation and cytokine production, even in PD-1-deficient T cells, suggesting additional receptors. Recent studies have concluded that PD-L2 also binds to a second receptor, repulsive guidance molecule b (RGMb), which was originally identified as a receptor for bone morphogenetic proteins (BMPs). RGMb is expressed in the central nervous system, as well as in macrophages, however, its role in immunity is only beginning to emerge. Interaction between PD-L2 and RGMb regulates the development of respiratory tolerance in the lung through BMP and/or neogenin signaling pathways. The naturally occurring human PD-L2 monomer consists of a 201-amino-acid extracellular domain, a 21-amino-acid transmembrane domain, and a 32-amino-acid cytoplasmic domain.

Specifications

• Antigen: CD273 (B7-DC) Chimeric
• Applications: ELISA, Flow Cytometry, Inhibition Assays, Immunoprecipitation, Western Blot
• Classification: Recombinant Monoclonal
• Clone: TY25
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.02% ProClin 300
• Gene: PDCD1LG2
• Gene Accession No.: Q9WUL5
• Gene Alias: B7 dendritic cell molecule; B7DC; B7-DC; bA574F11.2; Btdc; Butyrophilin B7-DC; butyrophilin-like protein; CD273; F730015O22Rik; MGC124039; MGC124040; PD1 ligand 2; PD-1 ligand 2; PD-1-ligand 2; PDCD1 ligand 2; PDCD1L2; Pdcd1lg2; Pdl2; PD-L2; Programmed cell death 1 ligand 2; programmed death ligand 2
• Gene Symbols: PDCD1LG2
• Host Species: Rabbit
• Immunogen: This antibody was raised by immunising SD rats with B7-DC-transfected RAW264.7 cells and selected on B7-DC-transfected NRK.
• Purification Method: Protein A
• Quantity: 200 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 58205
• Target Species: Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG κ