CD275 (B7-H2) Monoclonal Antibody (HK5.3), Biotin, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-120-94	Invitrogen™ 13598582	100 μg

Description

Description: The HK5.3 monoclonal antibody reacts with mouse B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of ∽40 kDa and belongs to the Ig superfamily. Mouse B7RP-1 is expressed by antigen presenting cells including B cells, monocytes/macrophages and dendritic cells. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway. Applications Reported: The HK5.3 antibody has been reported for use in flow cytometric analysis. HK5.3 is not recommended for use in immunohistochemical staining. Applications Tested: The HK5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS). ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function. ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue. Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.

Specifications

• Antigen: CD275 (B7-H2)
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: Q9JHJ8
• Gene Symbols: Icosl
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: Icosl

• Clone: HK5.3
• Applications: Flow Cytometry
• Conjugate: Biotin
• Host Species: Rat
• Target Species: Mouse
• Gene ID (Entrez): 50723
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.