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CD277 Mouse anti-Human, Brilliant Violet 786, Clone: 232-5, BD Optibuild™
Mouse Monoclonal Antibody
Supplier: BD Biosciences 742831
Description
The 232-5 monoclonal antibody specifically recognizes Butyrophilin 3 (BTN3), which is also known as CD277. BTN3 is comprised of three structurally related members, BTN3A1, BTN3A2, and BTN3A3. BTN3 molecules are transmembrane type 1 glycoproteins that belong to the Ig superfamily. The 232-5 antibody recognizes the variable region of the extracellular domains of BTN3 molecules. BTN3 molecules are differentially expressed by T, NKT, B, and NK cells, monocytes, macrophages, dendritic cells, as well as some stem cells and tumor cells. BTN3 expression is decreased upon lymphocyte activation. The extracellular domain of CD277 is structurally related to the B7 family of proteins that regulate T cell activation. A soluble form of the 232-5 antibody can serve as an agonist in BTN3-mediated regulation of anti-CD3±anti-CD28 antibody activated CD4+ or CD8+ T cells.
The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter). BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm. BV786 offers a bright choice for the 6th detector off the violet laser.
Specifications
| CD277 | |
| 232-5 | |
| Brilliant Violet 786 | |
| CD277, Butyrophilin 3, BTN3, BTN3A1, BTN3A2, BTN3A3, BTN3.1, BTN3.2, BTN3.3 | |
| Human BTN3A3 | |
| 50 μg | |
| Primary | |
| Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
| Monoclonal | |
| 0.2mg/mL | |
| Aqueous buffered solution containing ≤0.09% sodium azide. | |
| Mouse | |
| Affinity Purified | |
| RUO | |
| Human | |
| IgG2a κ |
Safety and Handling
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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