CD278 (ICOS) Monoclonal Antibody (7E.17G9), PE-Cyanine7, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-3475	Invitrogen™ 25994282	100 μg

Description

Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells. Applications Reported: This 7E.17G9 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 7E.17G9 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat.

CD278 (ICOS) Monoclonal antibody specifically detects CD278 (ICOS) in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD278 (ICOS)
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: Q9WVS0
• Isotype: IgG2b κ
• Purification Method: Affinity Chromatography
• Product Type: Antibody

• Clone: 7E.17G9
• Applications: Flow Cytometry
• Conjugate: PE-Cyanine7
• Host Species: Rat
• Target Species: Mouse
• Gene ID (Entrez): 54167
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!

For Research Use Only.