Invitrogen™ CD28 Monoclonal Antibody (1C6), Functional Grade, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 16028385

Catalog No. 50-112-9285

Description

Description: The 1C6 monoclonal antibody reacts with canine CD28, which is expressed by thymocytes, a majority of T cells, and a subset of NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells. The 1C6 antibody has been reported to have antagonist activity, as well as induce proliferation. The 1C6 and 5B8 antibodies recognize different epitopes of canine CD28. Applications Reported: This 1C6 antibody has been reported for use in flow cytometric analysis and functional assays. Proliferation assays with the 1C6 antibody are best performed using polypropylene plates. (Fluorochrome-conjugated 5B8 is recommended for use in flow cytometry.). Applications Tested: This 1C6 antibody has been tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

Specifications

• Antigen: CD28
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: 0
• Gene Symbols: Cd28
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 403646
• Content And Storage: 4°C
• Form: Liquid

• Applications: Flow Cytometry, Functional Assay
• Clone: 1C6
• Conjugate: Functional Grade
• Gene: Cd28
• Gene Alias: antigen CD28; CD28; CD28 antigen; CD28 antigen (Tp44); CD28 isoform; CD28 isoform 2; Cd28 molecule; CD28 precursor protein; CD28 protein; CD28 protein precursor; CD28RNA; cell surface protein; CHT28; contains partial extracellular domain; costimulatory molecule B7 receptor CD28; EGK_04709; MGC138290; sCD28; soluble CD28; T44; T-cell costimulatory molecule CD28; T-cell costimulatory molecule Tp44; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28 homolog; TP44
• Host Species: Mouse
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Canine
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.