CD28 Mouse Anti-Rat, Brilliant Violet 786, Clone: JJ319, BD Optibuild™

Catalog No.	Quantity
BDB742587	50 μg

Catalog No. BDB742587 Supplier BD Biosciences Supplier No. 742587

Description

Mouse Monoclonal Antibody

The JJ319 monoclonal antibody specifically recognizes CD28, which is expressed on virtually all T lymphocytes bearingαβT-cell receptors (TCR), on mostγδTCR-bearing T cells, and on a subset of NK cells. In the thymus, CD28 expression is developmentally regulated during the maturation ofαβTCR-bearing T cells. CD28 is a costimulatory receptor required for activation of T cells; its ligands include CD80 (B7-1) and CD86 (B7-2). Soluble JJ319 mAb costimulates the proliferative responses and IL-2 production of CD4+ and CD8+ T cells activated by anti-αβ-TCR mAb R73 (Cat. no. 554910). The alternate anti-rat CD28 mAb JJ316 (Cat. No. 554992) is capable of directly stimulating T cells in vitro and in vivo.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter). BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm. BV786 offers a bright choice for the 6th detector off the violet laser.

Specifications

• Antigen: CD28
• Classification: Monoclonal
• Clone: JJ319
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 786
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: Cd28, CD28RNA
• Host Species: Mouse
• Immunogen: Rat CD28-transfected cell line
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ