Invitrogen™ CD28 Recombinant Mouse Monoclonal Antibody (D665)

Catalog No.	Quantity
PIMA547870	200 μg

Catalog No. PIMA547870 Supplier Invitrogen™ Supplier No. MA547870

Description

Mouse Recombinant Monoclonal Antibody

Specificity: This antibody binds to the laterally exposed C D loop of CD28 and induces T cell proliferation without TCR ligation.

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

Specifications

• Antigen: CD28
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, T-Cell Activation, Surface Plasmon Resonance
• Classification: Recombinant Monoclonal
• Clone: D665
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.02% ProClin 300
• Gene: Cd28
• Gene Accession No.: P31041
• Gene Alias: antigen CD28; CD28; CD28 antigen; CD28 antigen (Tp44); CD28 isoform; CD28 isoform 2; Cd28 molecule; CD28 precursor protein; CD28 protein; CD28 protein precursor; CD28RNA; cell surface protein; CHT28; contains partial extracellular domain; costimulatory molecule B7 receptor CD28; EGK_04709; MGC138290; sCD28; soluble CD28; T44; T-cell costimulatory molecule CD28; T-cell costimulatory molecule Tp44; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28 homolog; TP44
• Gene Symbols: Cd28
• Host Species: Mouse
• Immunogen: The original D665 antibody was generated by immunizing mice alternately with A20 cells expressing mCD28 i.p. and with recombinant mCD28Ig s.c.
• Purification Method: Protein A
• Quantity: 200 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12487
• Target Species: Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ