CD284 (TLR4) Mouse anti-Human, Brilliant Violet 421, Clone: HTA125, BD Optibuild™

Catalog No.	Quantity
BDB743392	50 μg

Catalog No. BDB743392 Supplier BD Biosciences Supplier No. 743392

Description

Mouse Monoclonal Antibody

The HTA125 monoclonal antibody specifically binds to the human Toll-like receptor 4 (TLR4), also known as CD284. Toll-like receptors (TLR) are type I transmembrane proteins characterized by an extracellular domain containing leucine-rich repeats and a cytoplasmic domain similar to IL-1R family. In humans, ten TLR have been identified so far. The TLR family acts as a pattern-recognition receptor that is an essential component of pathogen recognition and innate immunity. TLR4 has been identified as the receptor for Gram-negative bacterial lipopolysaccharide (LPS). TLR4 mRNA expression has been found in spleen, placenta, ovary, intestine and lung. Using HTA125, TLR4 protein expression has been detected in monocytes, B cells and T cells in human peripheral blood. The human TLR4 /gene has been mapped to chromosome 9q32. The immunogen used to generate the HTA125 hybridoma was human TLR4-transfected Ba/F3 cells. The HTA125 antibody reportedly blocks LPS-triggered, TLR4-mediated stimulation of cells.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.

Specifications

• Antigen: CD284 (TLR4)
• Classification: Monoclonal
• Clone: HTA125
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 421
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: TLR4, CD284, Toll-like Receptor 4, ARMD10
• Host Species: Mouse
• Immunogen: Human TLR4-transfected Ba/F3 cell line
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a κ