CD29 (Integrin beta 1) Monoclonal Antibody (KMI6), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
14-029-281	Invitrogen™ 14029281	50 μg
50-125-11	Invitrogen™ 14029282	100 μg
50-125-12	Invitrogen™ 14029285	500 μg

Description

Description: The KMI6 monoclonal antibody reacts with mouse CD29 (integrin beta1), a 120-130 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the alpha integrins CD49a-f, to form the VLA-1 through VLA-6 complexes, respectively, and also with CD51. These alpha beta integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All of the integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins also interact with cellular receptors including VCAM-1 and MadCAM-1. The KMI6 antibody has been shown to be an activating antibody for beta1 integrins, enhancing lymphocyte binding to fibronectin. Applications Reported: This KMI6 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. The KMI6 antibody has also been found useful for immunoblotting, recognizing a protein of approximately 120 kD under non-reducing conditions. Applications Tested: This KMI6 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.125 μg per test.

ITGB1 (Integrin Subunit Beta 1, beta1 integrin subunit, GPIIa, CD29) is a 110 kDa cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. ITGB1 forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CDa-f) determining the binding properties of beta1 (VLA) integrins. ITGB1 is a cell adhesion molecule appearing on platelets, as the common Beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. Decreased expression of ITGB1 correlates with acquiring multidrug resistance of tumor cells in the presence of anti-tumor drug. ITGB1 is up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue. Further, ITGB1 also regulates tissue damage and disease symptoms related to inflammatory bowel disease. Through an ITGB1-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta. More than 8 beta subunits with numerous splice variant isoforms have been identified in mammals. There are two major forms of integrin beta1: beta1A and beta1D, which differ in 13 amino acids. The distribution pattern in adult tissues for integrin beta types are mutually exclusive. Beta1A is present in all tissues, except cardiac and skeletal muscle which express the beta1D variant.

Specifications

• Antigen: CD29 (Integrin beta 1)
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: 4633401G24Rik; AA409975; AA960159; Beta OL; beta oligodendroglia; CD29; CSAT antigen; ENSMUSG00000051907; Fibronectin receptor subunit beta; FNRB; Glycoprotein IIa; Gm9863; gpIIa; integrin beta 1; integrin beta 1 (fibronectin receptor beta); integrin beta 1A precursor; Integrin beta1; integrin beta-1; integrin beta-1 subunit; integrin beta1D; integrin precursor; integrin subunit beta 1; integrin VLA-4 beta subunit; integrin VLA-4 subunit beta; integrin, beta 1; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); ITBG1D; ITGB1; JG22 antigen; MDF2; MSK12; RGD-receptor; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLAB; VLA-BETA
• Gene Symbols: ITGB1
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: ITGB1

• Clone: KMI6
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: P09055
• Gene ID (Entrez): 16412
• Isotype: IgG2a κ
• Purification Method: Affinity Chromatography
• Product Type: Antibody

For Research Use Only.