CD294 (CRTH2) (extracellular) Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA5111888

Catalog No. PIPA5111888

Description

Reconstitute antibody with 50 μL of double distilled water (DDW). The reconstituted solution can be stored at 4 degrees C for up to 1 week. For longer periods, small aliquots should be stored at -20 degrees C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min). Peptide sequence is: (C)RAHSVTT LRQLASR Homology: Mouse amino acid residues are identical. Will not recognize DP2 receptor from human samples.

CRTH2 (GPR44) is a chemoattractant receptor-homologous molecule expressed on Th2 cells that is an identified receptor for the prostaglandin D2 (PGD2) and prostaglandin D receptor. CRTH2 is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses. However, CRTH2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the CRTH2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3. This suggests that CRTH2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo. CRTH2 is also a novel member of the G protein-coupled leukocyte chemoattractant receptor family, which is selectively expressed in Th2 but not Th1 lineage cells. CRTH2 has been reported to be expressed in the central nervous system, peripheral tissues, and in blood cells, particularly activated T helper-2 cells. Two transcripts have been isolated: a 3.5 kb transcript expressed primarily in the thalamus, frontal cortex, pons and hippocampus; and a 3.4 kb transcript expressed in fetal liver, leukocytes and thymus. CRTH2 is selectively expressed in vivo in an activated state of Th2 cells including allergen-responsive Th2 cells, suggesting its pivotal roles in ongoing Th2-type immune reactions. At least two different isoforms of CRTH2 are known to exist. Single nucleotide polymorphisms in the 3′ UTR of the CRTH2 gene have been associated with asthma susceptibility.

Specifications

• Antigen: CD294 (CRTH2) (extracellular)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PTGDR2
• Gene Alias: CD294; Chemoattractant receptor-homologous molecule expressed on Th2 cells; CRTH2; DL1R; DP2; G protein-coupled receptor 44; GPR44; G-protein coupled receptor 44; Grp45; MGC130436; Prostaglandin D2 receptor 2; PTGDR2
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 14764, 309212
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Western Blot
• Concentration: 0.8 mg/mL
• Formulation: PBS with 1% BSA and 0.05% sodium azide; pH 7.4
• Gene Accession No.: Q6XKD3, Q9Z2J6
• Gene Symbols: PTGDR2
• Immunogen: Peptide corresponding to amino acid residues 270-283 of rat PTGDR2 (3rd extracellular loop)
• Quantity: 50 μL
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG