CD294 (CRTH2) Rat anti-Human, PE-Cyanine5, Clone: BM16, eBioscience
Rat Monoclonal Antibody
Manufacturer: eBioscience 15294941
Description: This BM16 monoclonal antibody reacts with human CD294, which is also known as G protein-coupled receptor-44 (GPR-44) or chemoattractant receptor expressed on Th2 cells (CRTH2). CD294 is expressed on Th2 cells, some CD8+ cells, eosinophils, and basophils. Moreover, CD294 binds prostaglandin D2, which promotes leukocyte chemotaxis during allergic responses. CD294 has been reported to mediate prostaglandin D2-mediated suppression of apoptosis of Th2 cells by activating the PI3K pathway. Applications Reported: This BM16 antibody has been reported for use in flow cytometric analysis. Applications Tested: This BM16 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser The prostaglandin D2 receptor 2 (PTGDR2), also known as CRTH2, is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses. However, PTGDR2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the PTGDR2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3. This suggests that PTGDR2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo.
|PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2|
|4°C, store in dark, DO NOT FREEZE!|
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