Invitrogen™ CD2AP Monoclonal Antibody (5F8)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA533009

Catalog No. PIMA533009

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human K562 whole cell, human A431 whole cell, human HEK293 whole cell, human U20S whole cell, human HL-60 whole cell, human MCF-7 whole cell, human Hela whole cell, human PANC-1 whole cell, rat liver tissue, rat testicular tissue, mouse pancreas tissue, mouse testicular tissue, mouse RAW2467 whole cell. IHC: human mammary cancer, human mammary cancer, human colon cancer, human placenta tissue, mouse kidney tissue, rat brain tissue. ICC/IF: A431 cell. Flow: K562 cell, U20S cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease.

Specifications

• Antigen: CD2AP
• Classification: Monoclonal
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and 0.05mg sodium azide
• Gene Accession No.: F1LRS8, Q9JLQ0, Q9Y5K6
• Gene Symbols: CD2AP
• Immunogen: E.coli-derived human CD2AP recombinant protein (Position: K253-K337).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 5F8
• Conjugate: Unconjugated
• Gene: CD2AP
• Gene Alias: adapter protein CMS; AL024079; C78928; Cas ligand with multiple SH3 domains; Cas ligand with multiple Src homology 3 (SH3) domains; CD2 associated protein; Cd2ap; CD2-associated protein; CMS; mesenchyme to epithelium transition protein, SH3 domains; mesenchyme-to-epithelium transition protein with SH3 domains 1; Mets1; METS-1; SH3 domain-containing adapter protein; SH3 domain-containing adaptor protein METS-1
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12488, 23607, 316258
• Content And Storage: -20°C
• Form: Lyophilized