Invitrogen™ CD3/CD19 Antibody Cocktail, FITC, PE

Catalog No.	Quantity
PIMA516537	100 Tests

Catalog No. PIMA516537 Supplier Invitrogen™ Supplier No. MA516537

Description

Antibody Cocktail

MA5-16537 contains a monoclonal mixture of FITC-conjugated CD19 (clone #6D5, Rat IgG2a) and RPE-conjugated CD3 (clone #KT3, Rat IgG2a). This antibody is a dual-color reagent recognizing mouse CD19 and CD3. Clone 6D5 recognizes mouse CD19, a 95 kDa cell surface glycoprotein expressed by B lymphocytes. Clone KT3 recognizes mouse CD3, a cell surface glycoprotein expressed by mature T cells. Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL.

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lamda light chains. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation. It is expressed in all B cell precursor leukemias.

Specifications

• Antigen: CD3/CD19
• Applications: Flow Cytometry
• Classification: Cocktail
• Clone: 6D5/KT3
• Concentration: Conc. Not Determined
• Conjugate: FITC/PE
• Formulation: PBS with 1% BSA, 5% sucrose and 0.09% sodium azide
• Gene: CD3G
• Gene Accession No.: P04235, P11942, P22646, P24161, P25918
• Gene Alias: 4930549J05Rik; A430104F18Rik; AI504783; AW495831; AW552088; B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19; CD19 antigen; CD19 molecule; Cd247; CD247 antigen; CD247 antigen, zeta subunit; CD247 molecule; CD3; CD3 antigen, delta polypeptide; CD3 antigen, delta subunit; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 delta; CD3 epsilon; Cd3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule; CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3E; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule; CD3e molecule, epsilon (CD3-TCR complex); CD3epsilon; CD3-epsilon; Cd3-eta; CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule; CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3-GAMMA; CD3H; CD3Q; Cd3z; CD3Z antigen, zeta polypeptide (TiT3 complex); Cd3zeta; Cd3-zeta; CD3zeta chain; CD3-zeta/eta; Ctg3; Ctg-3; CVID3; Differentiation antigen CD19; FLJ18683; IMD17; IMD18; IMD19; IMD25; OKT3, delta chain; T3D; T3E; T3G; T3Z; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen Leu-12; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; TCR zeta chain; TCRE; Tcrk; Tcrz
• Gene Symbols: CD19, Cd247, CD3D, CD3E, CD3G
• Host Species: Rat
• Immunogen: Human K562 cell line transfected with murine CD19,.
• Purification Method: Purified
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12478, 12500, 12501, 12502, 12503
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG2a