CD3 epsilon Antibody (CRIS-7), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2448780.02MG

Catalog No. NBP24487800

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence

CD3 epsilon Monoclonal specifically detects CD3 epsilon in Human, Monkey samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence.

Specifications

• Antigen: CD3 epsilon
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence 1 - 2 ug/ml
• Gene Alias: CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3-epsilon, FLJ18683, T3E, T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, TCRE
• Host Species: Mouse
• Molecular Weight of Antigen: 20 kDa
• Quantity: 0.02 mg
• Research Discipline: Adaptive Immunity, Apoptosis, Cytokine Research, Diabetes Research, Immunology, Innate Immunity, Mesenchymal Stem Cell Markers, Signal Transduction, Stem Cell Lines, Stem Cell Markers
• Gene ID (Entrez): 916
• Target Species: Human, Monkey
• Form: Purified

• Applications: Flow Cytometry, Immunofluorescence
• Clone: CRIS-7
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Symbols: CD3E
• Immunogen: Stimulated human leukocytes
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes the -chain of CD3 (Workshop V; Code: CD03.09), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
• Content And Storage: Store at 4C.
• Isotype: IgG2a κ

For Research Use Only