Invitrogen™ CD3/HLA Class 2 Antibody Cocktail, FITC, PE

Catalog No.	Quantity
PIMA516958	50 Tests

Catalog No. PIMA516958 Supplier Invitrogen™ Supplier No. MA516958

Description

Antibody Cocktail

MA5-16958 contains a monoclonal mixture of FITC-conjugated CD3 (clone #UCH-T1, Mouse IgG1) and RPE-conjugated HLA Class 2 (clone #WR18, Mouse IgG2a). UCHT1 recognizes the human CD3 cell surface antigen expressed by T lymphocytes. WR18 recognizes a common determinant on the beta chain of the human HLA Class 2 molecules HLA-DR, DP and DQ. This antigen is expressed on B lymphocytes, monocytes and on activated T lymphocytes. This antibody is designed to permit the simultaneous enumeration of CD3 and HLA Class 2 expressing cells. It is particularly useful in the assessment of HLA Class 2 expression on T lymphocytes. Reconstitute with 0.5 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL.

The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined.

Specifications

• Antigen: CD3/HLA Class 2
• Applications: Flow Cytometry
• Classification: Cocktail
• Clone: UCHT1/WR18
• Concentration: Conc. Not Determined
• Conjugate: FITC/PE
• Formulation: PBS with 1% BSA, 5% sucrose and 0.09% sodium azide
• Gene: CD3G
• Gene Accession No.: P01911, P01920, P04234, P04440, P07766, P09693
• Gene Alias: beta1 domain MHC class II HLA DPB; CD3 antigen, delta subunit; CD3 delta; CD3 epsilon; CD3D; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule; CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3E; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule; CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; CD3G; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule; CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3-GAMMA; CD74 antigen; CELIAC1; class II HLA beta chain; Clone P2-beta-3; DHLAG; DPB1; DR-16; DRB1; DRw10; DW2.2/DR2.2; FLJ18683; HLA class II antigen; HLA class II histocompatibility antigen, DP beta 1 chain; HLA class II histocompatibility antigen, DP(W4) beta chain; HLA class II histocompatibility antigen, DQ beta 1 chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA class II histocompatibility antigen, DRB1-15 beta chain; HLA class II histocompatibility antigen, DRB1-16 beta chain; HLA class II histocompatibility antigen, DRB1-3 chain; HLA DP14-beta chain; HLADG; HLA-DP; HLA-DP histocompatibility type, beta-1 subunit; HLA-DP1B; HLA-DPB; HLA-DPB1; HLA-DQ; HLA-DQB; HLA-DQB1; HLA-DR1B; HLA-DRB; HLA-DRB1; HLA-DRB2; HLA-DR-gamma; human leucocyte antigen DRB1; IA-gamma; IDDM1; IMD17; IMD18; IMD19; lymphocyte antigen; lymphocyte antigen DRB1; major histocompatibility complex class II antigen beta chain; major histocompatibility complex, class II, DP beta 1; major histocompatibility complex, class II, DQ beta 1; major histocompatibility complex, class II, DR beta 1; MHC class II antigen; MHC class II antigen beta chain; MHC class II antigen DP beta 1 chain; MHC class II antigen DPB1; MHC class II antigen DPbeta1; MHC class II antigen DQB1; MHC class II antigen DRB1*15; MHC class II antigen DRB1*16; MHC class II antigen DRB1*3; MHC class II antigen HLA-DQ-beta-1; MHC class II DQ beta chain; MHC class II HLA-DP-beta-1; MHC class II HLA-DQ beta glycoprotein; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DRB1; MHC class II HLA-DR-beta cell surface glycoprotein; MHC class II HLA-DRw10-beta; MHC class2 antigen; MHC DQ beta; MHC HLA DPB1; OKT3, delta chain; SS1; T3D; T3E; T3G; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; TCRE
• Gene Symbols: CD3D, CD3E, CD3G, HLA-DPB1, HLA-DQB1, HLA-DRB1
• Host Species: Mouse
• Immunogen: Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome, Human B-CLL cells.
• Purification Method: Purified
• Quantity: 50 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3115, 3119, 3123, 915, 916, 917
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG1, IgG2a