CD3 Mouse anti-Human, Alexa Fluor 700, Clone: UCHT1, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 561027

Catalog No. BDB561027

Description

The UCHT1 monoclonal antibody specifically binds to the human CD3ε-chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3ε-positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3ε.

Specifications

• Antigen: CD3
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
• Quantity: 25 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: UCHT1
• Conjugate: Alexa Fluor 700
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG1 κ

For Research Use Only.