CD3 Mouse anti-Human, Brilliant Ultraviolet 737, Clone: SK7, BD Horizon™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB612753	BD Biosciences 612753	25 Tests
BDB612752	BD Biosciences 612752	100 Tests

Description

The SK7 (Leu-4) monoclonal antibody specifically binds to the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kDa. The antigen recognized by CD3 antibodies is noncovalently associated with either α / β or γ/ δ TCR (70 to 90 kDa). The CD3 antigen is present on 61% to 85% of normal peripheral blood lymphocytes 60% to 85% of thymocytes and on Purkinje cells in the cerebellum. The soluble form of this antibody has a mitogenic effect on most peripheral blood T lymphocytes, provided appropriate functional monocytes are present.

Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV737 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different BUV737 reagents (e.g., CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.

Specifications

• Antigen: CD3
• Applications: Flow Cytometry
• Conjugate: Brilliant Ultraviolet 737
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Isotype: IgG1 κ
• Purification Method: Affinity Purified

• Clone: SK7
• Classification: Monoclonal
• Host Species: Mouse
• Target Species: Human
• Immunogen: Human Thymocytes
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.