CD3 Mouse anti-Human, BUV395, Clone: UCHT1, BD

Catalog No.	Quantity
BDB563546	100 Tests
BDB563548	25 Tests

Catalog No. BDB563546 Supplier BD Biosciences Supplier No. 563546

Description

Mouse Monoclonal Antibody

The UCHT1 monoclonal antibody specifically binds to the human CD3 ε -chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3 ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3 ε .

The antibody was conjugated to BD Horizon™ BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355nm laser equipped instrument. With an Ex Max at 348nm and an Em Max at 395nm, this dye has virtually no spillover into any other detector. BD Horizon™ BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.

Specifications

• Antigen: CD3
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: UCHT1
• Conjugate: Brilliant Ultraviolet 395
• Description: CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor
• Purification Method: Affinity Purified
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ

For Research Use Only.