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CD3 Rat anti-Murine, Brilliant Ultraviolet 737, Clone: 17A2, BD Horizon™
Rat Monoclonal Antibody
Supplier: BD Biosciences 612803
Description
The 17A2 monoclonal antibody specifically binds to the T-cell receptor-associated CD3 complex that is expressed on many thymocytes and mature T lymphocytes. Plate-bound 17A2 antibody has been reported to induce IL-2 production by cultured T cells in the absence of accessory cells. The binding of the 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11. This suggests that the 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 antibody has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells.
The antibody was conjugated to BD Horizon BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm. BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter).
Specifications
CD3 | |
Monoclonal | |
0.2mg/mL | |
Aqueous buffered solution containing ≤0.09% sodium azide. | |
γδ TCR-positive T-T hybridoma D1 | |
50 μg | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
Flow Cytometry | |
17A2 | |
Brilliant Ultraviolet 737 | |
Rat | |
Affinity Purified | |
RUO | |
Mouse | |
IgG2b κ |
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