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CD30 Mouse anti-Human, Brilliant Violet 510, Clone: BerH8 (also known as Ber-H8), BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 744407

Catalog No. BDB744407

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The BerH8 monoclonal antibody specifically binds to CD30, a 120 kDa type I transmembrane glycoprotein expressed on stimulated T and B cells. CD30 is an activation marker initially identified to be expressed on Reed-Sternberg cells. It is also expressed on a few extrafollicular T and B cells located at the rim of germinal centers. CD30 serves as a cytokine receptor. It belongs to the nerve growth factor receptor/tumor necrosis factor receptor (NGFR/TNFR) superfamily and is also known as TNFRSF8. CD30 interaction with CD30 ligand (CD30L/CD153/TNFSF8) can mediate signals for proliferation, apoptosis and cytotoxicity of lymphoid cells.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.


Aqueous buffered solution containing ≤0.09% sodium azide.
TNFRSF8, TNR8, CD30L/CD153 receptor, Ki-1, D1S166E
Mouse IgG1, κ
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
BerH8 (also known as Ber-H8)
Brilliant Violet 510
Affinity Purified
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV510 under optimal conditions that minimize unconjugated dye and antibody.
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