CD301b (MGL2) Monoclonal Antibody (11A10-B7), eFluor™ 660, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-4333	Invitrogen™ 50301182	100 μg

Description

Description: The monoclonal antibody 11A10-B7 recognizes mouse CD301b. CD301b is also known as macrophage galactose-type C-type lectin 2 (MGL2). CD301b expression is restricted to dermal dendritic cells, connection tissue, and bone marrow derived dendritic cells. Macrophages also express CD301b at low levels. CD301b contains a single carbohydrate recognition domain that recognizes protein antigens that have a galactose and N-acetylgalactosamine (Gal/GalNAc). Gal/GalNAc protein antigens are efficiently internalized through CD301b and presented to antigen specific CD4+ cells via MHC Class II. CD301b+ dendritic cells are believed to play an important role in TH2 priming. Applications Reported: This 11A10-B7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 11A10-B7 antibody has been tested by flow cytometric analysis of mouse skin draining lymph node cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm).

CD301b is also known as macrophage galactose-type C-type lectin 2 (MGL2). CD301b expression is restricted to dermal dendritic cells, connection tissue, and bone marrow derived dendritic cells. Macrophages also express CD301b at low levels. CD301b contains a single carbohydrate recognition domain that recognizes protein antigens that have a galactose and N-acetylgalactosamine (Gal/GalNAc). Gal/GalNAc protein antigens are efficiently internalized through CD301b and presented to antigen specific CD4+ cells via MHC Class II. CD301b+ dendritic cells are believed to play an important role in TH2 priming.

Specifications

• Antigen: CD301b (MGL2)
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene ID (Entrez): 216864
• Isotype: IgG2a λ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: 11A10-B7
• Applications: Flow Cytometry
• Conjugate: eFluor 660
• Host Species: Rat
• Target Species: Mouse
• Gene Symbols: Mgl2
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: Mgl2

For Research Use Only.