Invitrogen™ CD305 (LAIR1) Monoclonal Antibody (NKTA255), PE, eBioscience™

Catalog No.	Quantity
50-245-298	100 Tests

Catalog No. 50-245-298 Supplier Invitrogen™ Supplier No. 12305942

Description

Mouse Monoclonal Antibody

Description: This NKTA255 monoclonal antibody reacts with human CD305, which is also known as leukocyte-associated Ig-like receptor (LAIR) 1. CD305 is a type I transmembrane glycoprotein that possesses an extracellular Ig-like domain and two intracellular ITIMs. CD305 is expressed on nearly all immune cells, including T, NK, and B cells, as well as monocytes, dendritic cells, eosinophils, basophils, and mast cells. In humans, this molecule is also expressed on CD34+ hematopoietic progenitor cells and thymocytes. Several isoforms of CD305 exist due to alternative splicing, and their expression may differ between cell types. As an inhibitory receptor, CD305 blocks cytokine-mediated signaling, cell proliferation, and apoptosis. Studies have also identified collagens as the ligands for CD305. The NKTA255 antibody has been reported to inhibit NK cytolytic activity. Applications Reported: This NKTA255 antibody has been reported for use in flow cytometric analysis. Applications Tested: This NKTA255 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: μ8 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufactur...

CD305, also known as LAIR1, is a type I transmembrane glycoprotein with an extracellular Ig-like domain and two intracellular ITIMs. It is expressed on a wide range of immune cells, including T, NK, and B cells, monocytes, dendritic cells, eosinophils, basophils, and mast cells. In humans, CD305 is also present on CD34+ hematopoietic progenitor cells and thymocytes. Several isoforms exist due to alternative splicing, with expression varying between cell types. CD305 functions as an inhibitory receptor, playing a key role in regulating immune responses. It blocks cytokine-mediated signaling, cell proliferation, and apoptosis. In NK, B, and T cells, CD305 modulates cytolytic functions and cytokine production, down-regulating IL2 and IFNG in CD4+ T cells while inducing transforming growth factor beta. In B cells, it reduces IgG and IgE production and decreases IL8, IL10, and TNF secretion. Activation of CD305 through tyrosine phosphorylation recruits phosphatases PTPN6 and PTPN11, although it may also function independently of SH2-containing phosphatases. It inhibits differentiation of peripheral blood precursors into dendritic cells and has been shown to induce apoptosis in myeloid leukemia cell lines. Collagens are identified as ligands for CD305, underscoring its role in immune regulation.

Specifications

• Antigen: CD305 (LAIR1)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: NKTA255
• Concentration: 5 μL/Test
• Conjugate: PE
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene: LAIR1
• Gene Accession No.: Q6GTX8
• Gene Alias: 5133400O11Rik; BB115266; CD305; D7Bwg0421e; immunoglobulin heavy chain variable region; Lair1; Lair-1; leukocyte associated immunoglobulin like receptor 1; leukocyte-associated Ig-like receptor 1; leukocyte-associated Ig-like receptor-1; leukocyte-associated immunoglobulin-like receptor 1; rLAIR1
• Gene Symbols: LAIR1
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3903
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1

Safety and Handling

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